STAGdb is a standardized, high-resolution SNP genotyping array and analysis workflow for identifying clonal genotypes (genets) in the coral genus Acropora and their dinoflagellate symbionts. The array simultaneously genotypes coral hosts and symbionts using bi-allelic SNP markers derived from Caribbean Acropora species and their dominant symbiont, Symbiodinium ‘fitti’. It enables discrimination of host populations, and determination of hybrid ancestry (sometimes referred to as “A. prolifera”). A subset of markers also allows genotyping of Pacific Acropora and detection of other symbionts to the genus level. STAG is implemented in a customized Galaxy platform, providing a standardized database of previously identified host genets and workflow for consistent genet identification, restoration planning, and downstream genomic analyses.
This Scientific Reports article describes the tool and database.
A github page for tracking user issues can be found here.
Users can join a listserve to receive updates after signing up for a STAG DB Galaxy account.